Genetic interaction mapping in mammalian cells using CRISPR interference.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28481362.
- Also identified by DOI 10.1038/nmeth.4286 and PMC identifier 5584685.
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Abstract
We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and comparison with protein-protein-interaction data revealed a functional map of chromatin regulation.
Medical subject headings
- Chromosome Mapping
- Clustered Regularly Interspaced Short Palindromic Repeats
- Epistasis, Genetic
- Protein Interaction Mapping