In Situ Nanopore Fabrication and Single-Molecule Sensing with Microscale Liquid Contacts.
basic_science · Level V
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- Record sourced from PubMed, PMID 28485922.
- Also identified by DOI 10.1021/acsnano.7b01519.
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Abstract
In this article, we introduce a flexible technique for high-throughput solid-state nanopore analysis of single biomolecules. By confining the electrolyte to a micron-scale liquid meniscus at the tip of a glass micropipette, we enable automation and reuse of a single solid-state membrane chip for measurements with hundreds of distinct nanopores per day. In addition to overcoming important experimental bottlenecks, the microscale liquid contact dramatically reduces device capacitance, which is a key limiting factor to the speed and fidelity of solid-state nanopore sensor recordings.