Nm-seq maps 2'-O-methylation sites in human mRNA with base precision.
basic_science · Level V
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- Record sourced from PubMed, PMID 28504680.
- Also identified by DOI 10.1038/nmeth.4294 and PMC identifier 5712428.
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Abstract
The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.
Medical subject headings
- RNA, Messenger