CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28581493.
- Also identified by DOI 10.1038/nmeth.4327.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
CRISPR-Cas9-induced DNA damage may have deleterious effects at high-copy-number genomic regions. Here, we use CRISPR base editors to knock out genes by changing single nucleotides to create stop codons. We show that the CRISPR-STOP method is an efficient and less deleterious alternative to wild-type Cas9 for gene-knockout studies. Early stop codons can be introduced in ∼17,000 human genes. CRISPR-STOP-mediated targeted screening demonstrates comparable efficiency to WT Cas9, which indicates the suitability of our approach for genome-wide functional screenings.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Codon, Terminator
- Gene Silencing