Differential analysis of RNA-seq incorporating quantification uncertainty.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28581496.
- Also identified by DOI 10.1038/nmeth.4324.
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Abstract
We describe sleuth (http://pachterlab.github.io/sleuth), a method for the differential analysis of gene expression data that utilizes bootstrapping in conjunction with response error linear modeling to decouple biological variance from inferential variance. sleuth is implemented in an interactive shiny app that utilizes kallisto quantifications and bootstraps for fast and accurate analysis of data from RNA-seq experiments.
Medical subject headings
- Computer Simulation
- Gene Expression
- RNA
- Software