Comparison of computational methods for Hi-C data analysis.

Forcato, Mattia; Nicoletti, Chiara; Pal, Koustav; Livi, Carmen Maria; Ferrari, Francesco; Bicciato, Silvio · Nat Methods · 2017

other · Level V

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Abstract

Hi-C is a genome-wide sequencing technique used to investigate 3D chromatin conformation inside the nucleus. Computational methods are required to analyze Hi-C data and identify chromatin interactions and topologically associating domains (TADs) from genome-wide contact probability maps. We quantitatively compared the performance of 13 algorithms in their analyses of Hi-C data from six landmark studies and simulations. This comparison revealed differences in the performance of methods for chromatin interaction identification, but more comparable results for TAD detection between algorithms.

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