webKnossos: efficient online 3D data annotation for connectomics.

Boergens, Kevin M; Berning, Manuel; Bocklisch, Tom; Bräunlein, Dominic; Drawitsch, Florian; Frohnhofen, Johannes; Herold, Tom; Otto, Philipp et al. · Nat Methods · 2017

basic_science · Level V

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Abstract

We report webKnossos, an in-browser annotation tool for 3D electron microscopic data. webKnossos provides flight mode, a single-view egocentric reconstruction method enabling trained annotator crowds to reconstruct at a speed of 1.5 ± 0.6 mm/h for axons and 2.1 ± 0.9 mm/h for dendrites in 3D electron microscopic data from mammalian cortex. webKnossos accelerates neurite reconstruction for connectomics by 4- to 13-fold compared with current state-of-the-art tools, thus extending the range of connectomes that can realistically be mapped in the future.

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