Visualizing adenosine-to-inosine RNA editing in single mammalian cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28604724.
- Also identified by DOI 10.1038/nmeth.4332 and PMC identifier 5542810.
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Abstract
Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about the biology of this conversion remain unknown because of a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine-edited transcripts in situ. We found that editing of the GRIA2, EIF2AK2, and NUP43 transcripts is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells, while GRIA2 editing levels vary.
Medical subject headings
- Adenosine
- In Situ Hybridization, Fluorescence
- Inosine
- Molecular Imaging
- Neurons
- RNA Editing