Adaptive optics improves multiphoton super-resolution imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28628128.
- Also identified by DOI 10.1038/nmeth.4337 and PMC identifier 7749710.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ∼250 μm from the coverslip surface.
Medical subject headings
- Image Enhancement
- Lenses
- Microscopy, Fluorescence, Multiphoton