Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking.
basic_science · Level V
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- Record sourced from PubMed, PMID 28683074.
- Also identified by DOI 10.1371/journal.pone.0180503 and PMC identifier 5500330.
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Abstract
A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. This work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.
Medical subject headings
- Fluorine Radioisotopes
- Positron-Emission Tomography
- Saccharomyces cerevisiae