Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28703221.
- Also identified by DOI 10.1038/ncomms15993 and PMC identifier 5511349.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.
Medical subject headings
- CRISPR-Cas Systems
- Chromatin Assembly and Disassembly