A tunable refractive index matching medium for live imaging cells, tissues and model organisms.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28708059.
- Also identified by DOI 10.7554/eLife.27240 and PMC identifier 5582871.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.
Medical subject headings
- Imaging, Three-Dimensional
- Refractometry
- Triiodobenzoic Acids