Conformational landscape of a virus by single-particle X-ray scattering.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28805793.
- Also identified by DOI 10.1038/nmeth.4395.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free electron laser, we determine the three-dimensional structure and conformational landscape of the PR772 virus to a detector-limited resolution of 9 nm. Our results indicate that a single conformational coordinate controls reorganization of the genome, growth of a tubular structure from a portal vertex and release of the genome. These results demonstrate that single-particle X-ray scattering has the potential to shed light on key biological processes.
Medical subject headings
- Algorithms
- Bacteriophages
- Crystallography, X-Ray
- DNA, Viral
- Imaging, Three-Dimensional
- Scattering, Small Angle