Massively parallel single-nucleus RNA-seq with DroNc-seq.
basic_science · Level V
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- Record sourced from PubMed, PMID 28846088.
- Also identified by DOI 10.1038/nmeth.4407 and PMC identifier 5623139.
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Abstract
Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human archived brain samples to demonstrate sensitive, efficient, and unbiased classification of cell types, paving the way for systematic charting of cell atlases.
Medical subject headings
- RNA
- Sequence Analysis, RNA