An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues.
basic_science · Level V
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- Record sourced from PubMed, PMID 28846090.
- Also identified by DOI 10.1038/nmeth.4396 and PMC identifier 5623106.
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Abstract
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.
Medical subject headings
- DNA
- Freezing
- Genome
- Specimen Handling