Multiplexed quantification of proteins and transcripts in single cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28854175.
- Also identified by DOI 10.1038/nbt.3973.
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Abstract
We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8<sup>+</sup> lymphocytes and to identify and characterize an unknown cell type.
Medical subject headings
- Proteins
- Single-Cell Analysis