Sampling strategies to capture single-cell heterogeneity.

Rajaram, Satwik; Heinrich, Louise E; Gordan, John D; Avva, Jayant; Bonness, Kathy M; Witkiewicz, Agnieszka K; Malter, James S; Atreya, Chloe E et al. · Nat Methods · 2017

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Abstract

Advances in single-cell technologies have highlighted the prevalence and biological significance of cellular heterogeneity. A critical question researchers face is how to design experiments that faithfully capture the true range of heterogeneity from samples of cellular populations. Here we develop a data-driven approach, illustrated in the context of image data, that estimates the sampling depth required for prospective investigations of single-cell heterogeneity from an existing collection of samples.

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