Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28869756.
- Also identified by DOI 10.1038/nmeth.4400 and PMC identifier 5678970.
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Abstract
Red-shifted bioluminescence reporters are desirable for biological imaging. We describe the development of red-shifted luciferins based on synthetic coelenterazine analogs and corresponding mutants of NanoLuc that enable bright bioluminescence. One pair in particular showed superior in vitro and in vivo sensitivity over commonly used bioluminescence reporters. We adapted this pair to develop a bioluminescence resonance-energy-based Antares reporter called Antares2, which offers improved signal from deep tissues.
Medical subject headings
- Firefly Luciferin
- Luciferases
- Luminescent Measurements