Inhibition of DYRK1A disrupts neural lineage specificationin human pluripotent stem cells.

Abstract

Genetic analysis has revealed that the dual specificity protein kinase DYRK1A has multiple roles in the development of the central nervous system. Increased <i>DYRK1A</i> gene dosage, such as occurs in Down syndrome, is known to affect neural progenitor cell differentiation, while haploinsufficiency of <i>DYRK1A</i> is associated with severe microcephaly. Using a set of known and newly synthesized DYRK1A inhibitors, along with CRISPR-mediated gene activation and shRNA knockdown of <i>DYRK1A</i>, we show here that chemical inhibition or genetic knockdown of <i>DYRK1A</i> interferes with neural specification of human pluripotent stem cells, a process equating to the earliest stage of human brain development. Specifically, DYRK1A inhibition insulates the self-renewing subpopulation of human pluripotent stem cells from powerful signals that drive neural induction. Our results suggest a novel mechanism for the disruptive effects of the absence or haploinsufficiency of <i>DYRK1A</i> on early mammalian development, and reveal a requirement for <i>DYRK1A</i> in the acquisition of competence for differentiation in human pluripotent stem cells.