Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody.
basic_science · Level V
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- Record sourced from PubMed, PMID 28910287.
- Also identified by DOI 10.1371/journal.pone.0183315 and PMC identifier 5598927.
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Abstract
Because of the broad neutralization and in vivo protection across influenza A and influenza B virus strains, monoclonal antibody CR9114 is widely used in influenza virus research as a positive control in many experiments. To produce amounts sufficient for the demand requires regular transient transfections, resulting in varying yield as well as differing batch to batch quality. Here, we report the development of a serum-free CHO DG44 cell line, stably producing a CR9114-like antibody with a potential to become a useful influenza virus research tool.
Medical subject headings
- Antibodies, Neutralizing
- CHO Cells
- Cell Culture Techniques
- Influenza B virus