Measuring the effects of particle orientation to improve the efficiency of electron cryomicroscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28931821.
- Also identified by DOI 10.1038/s41467-017-00782-3 and PMC identifier 5607000.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The orientation distribution of a single-particle electron cryomicroscopy specimen limits the resolution of the reconstructed density map. Here we define a statistical quantity, the efficiency, E <sub>od</sub>, which characterises the orientation distribution via its corresponding point spread function. The efficiency measures the ability of the distribution to provide uniform information and resolution in all directions of the reconstruction, independent of other factors. This metric allows rapid and rigorous evaluation of specimen preparation methods, assisting structure determination to high resolution with minimal data.A number of parameters influence the resolution of a cryo-EM structure. Here the authors investigate the effects of specimen orientation in single particle cryo-EM and present open-source software for rapidly assessing orientation distributions to improve data collection.
Medical subject headings
- Cryoelectron Microscopy
- Imaging, Three-Dimensional
- Ribosome Subunits