Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28991891.
- Also identified by DOI 10.1038/nmeth.4461 and PMC identifier 5679434.
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Abstract
Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules.
Medical subject headings
- Cryoelectron Microscopy
- Macromolecular Substances