SCENIC: single-cell regulatory network inference and clustering.

Aibar, Sara; González-Blas, Carmen Bravo; Moerman, Thomas; Huynh-Thu, Vân Anh; Imrichova, Hana; Hulselmans, Gert; Rambow, Florian; Marine, Jean-Christophe et al. · Nat Methods · 2017

basic_science · Level V

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Abstract

We present SCENIC, a computational method for simultaneous gene regulatory network reconstruction and cell-state identification from single-cell RNA-seq data (http://scenic.aertslab.org). On a compendium of single-cell data from tumors and brain, we demonstrate that cis-regulatory analysis can be exploited to guide the identification of transcription factors and cell states. SCENIC provides critical biological insights into the mechanisms driving cellular heterogeneity.

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