Expression level of human TLR4 rather than sequence is the key determinant of LPS responsiveness.
basic_science · Level V
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- Record sourced from PubMed, PMID 29020088.
- Also identified by DOI 10.1371/journal.pone.0186308 and PMC identifier 5636155.
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Abstract
To address the role of Toll-like receptor 4 (TLR4) single nucleotide polymorphisms (SNP) in lipopolysaccharide (LPS) recognition, we generated mice that differed only in the sequence of TLR4. We used a bacterial artificial chromosome (BAC) transgenic approach and TLR4/MD-2 knockout mice to specifically examine the role of human TLR4 variants in recognition of LPS. Using in vitro and in vivo assays we found that the expression level rather than the sequence of TLR4 played a larger role in recognition of LPS, especially hypoacylated LPS.
Medical subject headings
- Lipopolysaccharides
- Toll-Like Receptor 4