Antibodies to biotin enable large-scale detection of biotinylation sites on proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29039416.
- Also identified by DOI 10.1038/nmeth.4465 and PMC identifier 8954634.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide mixtures. Live-cell proximity labeling using APEX peroxidase followed by anti-biotin enrichment and mass spectrometry yielded over 1,600 biotinylation sites on hundreds of proteins, an increase of more than 30-fold in the number of biotinylation sites identified compared to streptavidin-based enrichment of proteins.
Medical subject headings
- Antibodies
- Biotin
- Peptides
- Proteins