Rapid nonlinear image scanning microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29039418.
- Also identified by DOI 10.1038/nmeth.4467.
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Abstract
Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.
Medical subject headings
- Microscopy, Confocal
- Microscopy, Fluorescence