High-throughput, image-based screening of pooled genetic-variant libraries.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29083401.
- Also identified by DOI 10.1038/nmeth.4495 and PMC identifier 5958624.
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Abstract
We report a high-throughput screening method that allows diverse genotypes and corresponding phenotypes to be imaged in individual cells. We achieve genotyping by introducing barcoded genetic variants into cells as pooled libraries and reading the barcodes out using massively multiplexed fluorescence in situ hybridization. To demonstrate the power of image-based pooled screening, we identified brighter and more photostable variants of the fluorescent protein YFAST among 60,000 variants.
Medical subject headings
- DNA Barcoding, Taxonomic
- Escherichia coli
- Gene Library
- High-Throughput Nucleotide Sequencing
- In Situ Hybridization, Fluorescence