Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29083402.
- Also identified by DOI 10.1038/nmeth.4483 and PMC identifier 5909187.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Targeted and inducible regulation of mammalian gene expression is a broadly important capability. We engineered drug-inducible catalytically inactive Cpf1 nuclease fused to transcriptional activation domains to tune the expression of endogenous genes in human cells. Leveraging the multiplex capability of the Cpf1 platform, we demonstrate both synergistic and combinatorial gene expression in human cells. Our work should enable the development of multiplex gene perturbation library screens for understanding complex cellular phenotypes.
Medical subject headings
- Bacterial Proteins
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Endonucleases
- Transcriptional Activation