Quantitative 3D-imaging for cell biology and ecology of environmental microbial eukaryotes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29087936.
- Also identified by DOI 10.7554/eLife.26066 and PMC identifier 5663481.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We present a 3D-fluorescence imaging and classification tool for high throughput analysis of microbial eukaryotes in environmental samples. It entails high-content feature extraction that permits accurate automated taxonomic classification and quantitative data about organism ultrastructures and interactions. Using plankton samples from the <i>Tara</i> Oceans expeditions, we validate its applicability to taxonomic profiling and ecosystem analyses, and discuss its potential for future integration of eukaryotic cell biology into evolutionary and ecological studies.
Medical subject headings
- Ecosystem
- Environmental Microbiology
- Eukaryota
- Imaging, Three-Dimensional
- Optical Imaging