Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29123102.
- Also identified by DOI 10.1038/s41467-017-01752-5 and PMC identifier 5680258.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.
Medical subject headings
- Endocytosis
- Exocytosis
- Fluorescent Dyes