Profiling the transcriptome with RNA SPOTs.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29131163.
- Also identified by DOI 10.1038/nmeth.4500 and PMC identifier 5819366.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Single-molecule FISH (smFISH) has been the gold standard for quantifying individual transcript abundances. Here, we scale up multiplexed smFISH to the transcriptome level and profile 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This method, called RNA sequential probing of targets (SPOTs), provides an accurate, flexible, and low-cost alternative to sequencing for profiling transcriptomes.
Medical subject headings
- Gene Expression Profiling
- In Situ Hybridization, Fluorescence
- RNA Probes
- RNA, Messenger