DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29162844.
- Also identified by DOI 10.1038/s41467-017-01891-9 and PMC identifier 5698298.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Progress in whole-genome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new "nanomapping" method to detect and map precisely BCL2-IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM "nanomapping" technique can be complementary to both sequencing and other physical mapping approaches.
Medical subject headings
- CRISPR-Cas Systems
- Chromosome Mapping
- DNA
- Genomics
- Nanoparticles