Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29167440.
- Also identified by DOI 10.1038/s41467-017-01650-w and PMC identifier 5700056.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The bacteria-derived clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are powerful tools for genome engineering. Recently, in addition to Cas protein engineering, the improvement of guide RNAs are also performed, contributing to broadening the research area of CRISPR-Cas9 systems. Here we develop a fusion guide RNA (fgRNA) that functions with both Cas9 and Cpf1 proteins to induce mutations in human cells. Furthermore, we demonstrate that fgRNAs can be used in multiplex genome editing and orthogonal genome manipulation with two types of Cas proteins. Our results show that fgRNAs can be used as a tool for performing multiple gene manipulations.
Medical subject headings
- Bacterial Proteins
- CRISPR-Cas Systems
- Endonucleases
- Genetic Engineering
- RNA, Guide, CRISPR-Cas Systems