Flow cytometric discrimination of seven lineage markers by using two fluorochromes.
basic_science · Level V
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- Record sourced from PubMed, PMID 29190813.
- Also identified by DOI 10.1371/journal.pone.0188916 and PMC identifier 5708788.
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Abstract
Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.
Medical subject headings
- Biomarkers
- Flow Cytometry
- Fluorescent Dyes
- Lymphocytes