Structural insight into catalytic mechanism of PET hydrolase.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29235460.
- Also identified by DOI 10.1038/s41467-017-02255-z and PMC identifier 5727383.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.
Medical subject headings
- Bacterial Proteins
- Burkholderiales
- Hydrolases
- Polyethylene Terephthalates