Labeling and identifying cell-specific proteomes in the mouse brain.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29251727.
- Also identified by DOI 10.1038/nbt.4056 and PMC identifier 6010150.
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Abstract
We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.
Medical subject headings
- Amino Acyl-tRNA Synthetases
- Brain
- Neurons
- Proteome