Myeloid cell recruitment versus local proliferation differentiates susceptibility from resistance to filarial infection.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29299998.
- Also identified by DOI 10.7554/eLife.30947 and PMC identifier 5754202.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Both T<sub>H</sub>2-dependent helminth killing and suppression of the T<sub>H</sub>2 effector response have been attributed to macrophages (MΦ) activated by IL-4 (M(IL-4)). To investigate how M(IL-4) contribute to diverse infection outcomes, the MΦ compartment of susceptible BALB/c mice and more resistant C57BL/6 mice was profiled during infection of the pleural cavity with the filarial nematode, <i>Litomosoides sigmodontis.</i> C57BL/6 mice exhibited a profoundly expanded resident MΦ (resMΦ) population, which was gradually replenished from the bone marrow in an age-dependent manner. Infection status did not alter the bone-marrow derived contribution to the resMΦ population, confirming local proliferation as the driver of resMΦ expansion. Significantly less resMΦ expansion was observed in the susceptible BALB/c strain, which instead exhibited an influx of monocytes that assumed an immunosuppressive PD-L2<sup>+</sup> phenotype. Inhibition of monocyte recruitment enhanced nematode killing. Thus, the balance of monocytic vs. resident M(IL-4) numbers varies between inbred mouse strains and impacts infection outcome.
Medical subject headings
- Cell Movement
- Cell Proliferation
- Filariasis
- Filarioidea
- Macrophages