Functional interrogation and mining of natively paired human V<sub>H</sub>:V<sub>L</sub> antibody repertoires.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29309060.
- Also identified by DOI 10.1038/nbt.4052 and PMC identifier 5801115.
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Abstract
We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (V<sub>H</sub>:V<sub>L</sub>) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.
Medical subject headings
- Antibodies, Neutralizing
- B-Lymphocytes
- HIV Antibodies
- HIV Infections