Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29330365.
- Also identified by DOI 10.1038/s41467-017-02261-1 and PMC identifier 5766611.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Extracellular vesicles (EVs) carry RNA, DNA, proteins, and lipids. Specifically, tumor-derived EVs have the potential to be utilized as disease-specific biomarkers. However, a lack of methods to isolate tumor-specific EVs has limited their use in clinical settings. Here we report a sensitive analytical microfluidic platform (<sup>EV</sup>HB-Chip) that enables tumor-specific EV-RNA isolation within 3 h. Using the <sup>EV</sup>HB-Chip, we achieve 94% tumor-EV specificity, a limit of detection of 100 EVs per μL, and a 10-fold increase in tumor RNA enrichment in comparison to other methods. Our approach allows for the subsequent release of captured tumor EVs, enabling downstream characterization and functional studies. Processing serum and plasma samples from glioblastoma multiforme (GBM) patients, we can detect the mutant EGFRvIII mRNA. Moreover, using next-generation RNA sequencing, we identify genes specific to GBM as well as transcripts that are hallmarks for the four genetic subtypes of the disease.
Medical subject headings
- Brain Neoplasms
- Extracellular Vesicles
- Glioblastoma
- Microfluidics