The lncRNA GATA6-AS epigenetically regulates endothelial gene expression via interaction with LOXL2.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29339785.
- Also identified by DOI 10.1038/s41467-017-02431-1 and PMC identifier 5770451.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Impaired or excessive growth of endothelial cells contributes to several diseases. However, the functional involvement of regulatory long non-coding RNAs in these processes is not well defined. Here, we show that the long non-coding antisense transcript of GATA6 (GATA6-AS) interacts with the epigenetic regulator LOXL2 to regulate endothelial gene expression via changes in histone methylation. Using RNA deep sequencing, we find that GATA6-AS is upregulated in endothelial cells during hypoxia. Silencing of GATA6-AS diminishes TGF-β2-induced endothelial-mesenchymal transition in vitro and promotes formation of blood vessels in mice. We identify LOXL2, known to remove activating H3K4me3 chromatin marks, as a GATA6-AS-associated protein, and reveal a set of angiogenesis-related genes that are inversely regulated by LOXL2 and GATA6-AS silencing. As GATA6-AS silencing reduces H3K4me3 methylation of two of these genes, periostin and cyclooxygenase-2, we conclude that GATA6-AS acts as negative regulator of nuclear LOXL2 function.
Medical subject headings
- Amino Acid Oxidoreductases
- Endothelial Cells
- GATA6 Transcription Factor
- Gene Expression Regulation
- Hypoxia
- Neovascularization, Physiologic
- RNA, Antisense
- RNA, Long Noncoding