X-ray scattering reveals molecular tilt is an order parameter for the main phase transition in a model biomembrane.

Abstract

Synchrotron diffuse x-ray scattering data reveal a dramatic softening of the molecular tilt modulus K_{θ} of the model biomembrane composed of DMPC lipids as the temperature is lowered towards the main phase transition temperature at T_{M}=24^{∘}C. Spontaneous tilt occurs below T_{M}, suggesting that tilt is a symmetry breaking order parameter. Consistent with this hypothesis, it is also found that a different lipid POPS has no spontaneous tilt below its T_{M} at 14^{∘}C and correspondingly its tilt modulus did not soften as T_{M} was approached from above. As previously known, the bending modulus K_{C} of DMPC also softens close to T_{M}, but unlike the tilt modulus, K_{C} has a maximum 3^{∘} above T_{M}, which also marks the limit of the well-known anomalous swelling regime. Tilt adds a different perspective to our previous understanding of the main phase transition in lipid bilayers.