TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29355846.
- Also identified by DOI 10.1038/nmeth.4582 and PMC identifier 5831505.
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Abstract
RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.
Medical subject headings
- Cytidine
- High-Throughput Nucleotide Sequencing
- Sequence Analysis, RNA
- Thiouridine
- Transcriptome