Precision of CAPILLARYS 2 for the Detection of Hemoglobin Variants Based on Their Migration Positions.
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- Record sourced from PubMed, PMID 29365076.
- Also identified by DOI 10.1093/ajcp/aqx148.
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Abstract
In this report, we evaluated utility of the capillary electrophoresis (CE) migration position of the CAPILLARYS 2 CE instrument. The precision of this x-axis number was determined on a selection of common hemoglobin (Hb) variants (Hb S, Hb C, Hb D-Punjab, Hb E, Hb Hope), and the reproducibility of this number was evaluated by comparing the results obtained by two large reference laboratories on 81 Hb variants. Additionally, the CE migration position is given for a total of 409 Hb variants. The x-axis migration position showed excellent intra- and interassay precision. Comparison of Hb variants seen by both laboratories showed that 83% had a difference in migration position of 1 unit or less. Only three rare Hb variants showed a difference of more than 2 units. In summary, the CE migration position is a reproducible value and can be used as an aid in the identification of Hb variants.
Medical subject headings
- Data Accuracy
- Electrophoresis, Capillary
- Hemoglobins