LARP7 family proteins have conserved function in telomerase assembly.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29422501.
- Also identified by DOI 10.1038/s41467-017-02296-4 and PMC identifier 5805788.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Understanding the intricacies of telomerase regulation is crucial due to the potential health benefits of modifying its activity. Telomerase is composed of an RNA component and reverse transcriptase. However, additional factors required during biogenesis vary between species. Here we have identified fission yeast Lar7 as a member of the conserved LARP7 family, which includes the Tetrahymena telomerase-binding protein p65 and human LARP7. We show that Lar7 has conserved RNA-recognition motifs, which bind telomerase RNA to protect it from exosomal degradation. In addition, Lar7 is required to stabilise the association of telomerase RNA with the protective complex LSm2-8, and telomerase reverse transcriptase. Lar7 remains a component of the mature telomerase complex and is required for telomerase localisation to the telomere. Collectively, we demonstrate that Lar7 is a crucial player in fission yeast telomerase biogenesis, similarly to p65 in Tetrahymena, and highlight the LARP7 family as a conserved factor in telomere maintenance.
Medical subject headings
- Nuclear Proteins
- Phosphoproteins
- Protozoan Proteins
- RNA
- RNA, Fungal
- RNA-Directed DNA Polymerase
- Ribonucleoproteins
- Schizosaccharomyces
- Telomerase