CellCycleTRACER accounts for cell cycle and volume in mass cytometry data.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29434325.
- Also identified by DOI 10.1038/s41467-018-03005-5 and PMC identifier 5809393.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Recent studies have shown that cell cycle and cell volume are confounding factors when studying biological phenomena in single cells. Here we present a combined experimental and computational method, CellCycleTRACER, to account for these factors in mass cytometry data. CellCycleTRACER is applied to mass cytometry data collected on three different cell types during a TNFα stimulation time-course. CellCycleTRACER reveals signaling relationships and cell heterogeneity that were otherwise masked.
Medical subject headings
- Cell Cycle
- Cells
- Flow Cytometry
- Single-Cell Analysis