Hydrogen peroxide positively regulates brassinosteroid signaling through oxidation of the BRASSINAZOLE-RESISTANT1 transcription factor.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29540799.
- Also identified by DOI 10.1038/s41467-018-03463-x and PMC identifier 5852159.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is an important signaling molecule in plant developmental processes and stress responses. However, whether H<sub>2</sub>O<sub>2</sub>-mediated signaling crosstalks with plant hormone signaling is largely unclear. Here, we show that H<sub>2</sub>O<sub>2</sub> induces the oxidation of the BRASSINAZOLE-RESISTANT1 (BZR1) transcription factor, which functions as a master regulator of brassinosteroid (BR) signaling. Oxidative modification enhances BZR1 transcriptional activity by promoting its interaction with key regulators in the auxin-signaling and light-signaling pathways, including AUXIN RESPONSE FACTOR6 (ARF6) and PHYTOCHROME INTERACTING FACTOR4 (PIF4). Genome-wide analysis shows that H<sub>2</sub>O<sub>2</sub>-dependent regulation of BZR1 activity plays a major role in modifying gene expression related to several BR-mediated biological processes. Furthermore, we show that the thioredoxin TRXh5 can interact with BZR1 and catalyzes its reduction. We conclude that reversible oxidation of BZR1 connects H<sub>2</sub>O<sub>2</sub>-mediated and thioredoxin-mediated redox signaling to BR signaling to regulate plant development.
Medical subject headings
- Arabidopsis Proteins
- Brassinosteroids
- Hydrogen Peroxide
- Nuclear Proteins