Axial Colocalization of Single Molecules with Nanometer Accuracy Using Metal-Induced Energy Transfer.
basic_science · Level V
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- Record sourced from PubMed, PMID 29562123.
- Also identified by DOI 10.1021/acs.nanolett.8b00425.
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Abstract
Single-molecule localization based super-resolution microscopy has revolutionized optical microscopy and routinely allows for resolving structural details down to a few nanometers. However, there exists a rather large discrepancy between lateral and axial localization accuracy, the latter typically three to five times worse than the former. Here, we use single-molecule metal-induced energy transfer (smMIET) to localize single molecules along the optical axis, and to measure their axial distance with an accuracy of 5 nm. smMIET relies only on fluorescence lifetime measurements and does not require additional complex optical setups.