Antiadhesive and cytotoxic effect of Iranian <i>Vipera lebetina</i> snake venom on lung epithelial cancer cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 29564263.
- Also identified by DOI 10.4103/jfmpc.jfmpc_208_17 and PMC identifier 5848398.
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Abstract
Cancer is one of the major health problems worldwide. Hence, finding potent therapeutics from natural sources seems necessary. Snake venom of <i>Vipera lebetina</i> contains potential component with anticancer activities such as antiproliferation, migration, invasion, adhesion, and angiogenesis effect. Evaluation of cytotoxic and antiadhesive effect of <i>V. lebetina</i> venom on lung epithelial cancer tumor cell (TC-1) was the main aim of this study. Here, we purified snake venom of <i>V. lebetina</i> by fast protein liquid chromatography (FPLC) using Sephacryl S-200 hr column. The fractions collected and evaluated by SDS-PAGE analysis. The cytotoxicity and antiadhesive effect of crude venom and fractions on TC-1 cells were demonstrated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and adhesion assay, respectively. Our results showed six fractions in FPLC diagram. <i>V. lebetina</i> crude venom and fractions showed dose-dependent cytotoxic effect on TC-1 cells. Fractions 2 and 5 showed high cytotoxic effect with high IC50 value (IC50 = 6 μg/ml for fraction 2 and IC50 = 7.3 μg/ml for fraction 5). Fractions 2 and 5 selected for analysis antiadhesive effect on TC-1 cells. Furthermore, our results showed that both fractions 2 and 5 had antiadhesive effect on TC-1 cells. Because of potent cytotoxic and antiadhesive effect of <i>V. lebetina</i> fractions on lung epithelial cancer cell line, it could be promising tools for further analysis as anticancer therapeutic development.