Exploring the anti-quorum sensing activity of a d-limonene nanoemulsion for Escherichia coli O157:H7.

Wang, Renjie; Vega, Pablo; Xu, Yi; Chen, Chin-Yi; Irudayaraj, Joseph · J Biomed Mater Res A · 2018

basic_science · Level V

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Abstract

In this study, a d-limonene nanoemulsion was developed by using a spontaneous emulsification method and its potential to inhibit the quorum sensing (QS)-regulated properties of Escherichia coli O157:H7 (E. coli) were revealed. The results in this study showed that d-limonene nanoemulsion inhibited E. coli biofilm formation through the suppression of curli and extracellular polymeric substance (EPS) production without inhibiting cell growth, and decreased swimming and swarming ability. Further analyses showed that d-limonene nanoemulsion interfered with auto-inducer 2 (AI-2) communication and repressed the expression of curli related genes and AI-2 importer genes in E. coli. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1979-1986, 2018.

Medical subject headings