Long-term in vivo recording of circadian rhythms in brains of freely moving mice.
basic_science · Level V
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- Record sourced from PubMed, PMID 29610316.
- Also identified by DOI 10.1073/pnas.1717735115 and PMC identifier 5910830.
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Abstract
Endogenous circadian clocks control 24-h physiological and behavioral rhythms in mammals. Here, we report a real-time in vivo fluorescence recording system that enables long-term monitoring of circadian rhythms in the brains of freely moving mice. With a designed reporter of circadian clock gene expression, we tracked robust <i>Cry1</i> transcription reporter rhythms in the suprachiasmatic nucleus (SCN) of WT, <i>Cry1</i><sup><i>-/-</i></sup> , and <i>Cry2</i><sup><i>-/-</i></sup> mice in LD (12 h light, 12 h dark) and DD (constant darkness) conditions and verified that signals remained stable for over 6 mo. Further, we recorded <i>Cry1</i> transcriptional rhythms in the subparaventricular zone (SPZ) and hippocampal CA1/2 regions of WT mice housed under LD and DD conditions. By using a Cre-loxP system, we recorded <i>Per2</i> and <i>Cry1</i> transcription rhythms specifically in vasoactive intestinal peptide (VIP) neurons of the SCN. Finally, we demonstrated the dynamics of <i>Per2</i> and <i>Cry1</i> transcriptional rhythms in SCN VIP neurons following an 8-h phase advance in the light/dark cycle.
Medical subject headings
- Circadian Rhythm
- Cryptochromes
- Fiber Optic Technology
- Fluorometry
- Neurons
- Period Circadian Proteins
- Suprachiasmatic Nucleus